Rabbit Nerve Growth Factor (NGF) ELISA Kit

SKU: orb782598

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nerve Growth Factor(NGF).

Research Area

Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit NGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit NGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit NGF in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rabbit
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 11.2 pg/mL
• Concentration: 2000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

NGFB ELISA Kit, Beta-NGF ELISA Kit, HSAN5 ELISA Kit, NGF-B ELISA Kit, Beta-Nerve Growth Factor ELISA Kit