Rabbit Matrix Metalloproteinase 3 (MMP3) ELISA Kit

SKU: orb775721

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 3(MMP3).

Research Area

Cancer Biology, Cardiovascular Research, Infectious Disease & Virology, Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit MMP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit MMP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit MMP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit MMP3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Rabbit
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.113 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

SL1 ELISA Kit, STMY ELISA Kit, STMY1 ELISA Kit, STR1 ELISA Kit, Progelatinase ELISA Kit, Stromelysin 1 ELISA Kit, Transin-1 ELISA Kit

Quick Database Links

UniProt

P28863