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| Catalog Number | orb1173550 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysosomal Associated Membrane Protein 2(LAMP2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lysosomal Associated Membrane Protein 2(LAMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysosomal Associated Membrane Protein 2(LAMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysosomal Associated Membrane Protein 2(LAMP2) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Rabbit |
| Concentration | 4000 pg/mL |
| Tested applications | ELISA |
| Application notes | standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit LAMP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit LAMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit LAMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit LAMP2 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 62.5-4000 pg/mL |
| Sensitivity | 23.7 pg/mL |
| Sample Types | Serum, plasma and other biological fluids |
| Alternative names | CD107-B, CD107b, LAMP-2C, LAMPB, LGP96, Lysosome-a Read more... |
| Note | For research use only |
