Rabbit IgG

• Catalog Number: orb570250
• Category: Proteins
• Description: Rabbit IgG
• Conjugation: Unconjugated
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01M PBS (pH7.4).
• Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: Rabbit Immunoglobulin G; Normal Rabbit IgG; Native
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: NIH/3T3. Isotype control: Rabbit IgG (orb570250), dilution: 1 ug/Test, Primary Antibody (green line): Rabbit Anti-beta-Actin antibody, dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Isotype control antibody (orange line) was rabbit IgG. The secondary antibody (blue line) used for 40 min at room temperature. Acquisition of 20000 events was performed.