Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

• Catalog Number: orb347719
• Category: Antibodies
• Description: RABBIT IgG (H&L) antibody (Alkaline Phosphatase)
• Species/Host: Guinea pig
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Rabbit
• Isotype: IgG
• Immunogen: Rabbit IgG whole molecule
• Antibody Type: Secondary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Guinea Pig Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human and Mouse Serum Proteins.
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Guinea Pig Anti-Rabbit IgG Alkaline Phosphatase Co
• Note: For research use only
• Application notes: This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western Blot of Anti-Rabbit IgG (H&L) (GUINEA PIG) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n orb347716). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n orb2652747). Lane 2: Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GUINEA PIG) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n orb347716) 1:500 for 60 min at RT. Secondary antibody: Anti-Guinea Pig IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347053) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab). Rabbit F(c) migrates slightly higher.