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Rabbit IgG F(ab')2 Agarose Antibody

SKU: orb346308

Description

Rabbit IgG F(ab')2 Agarose Antibody

Research Area

Molecular Biology

Images & Validation

Tested ApplicationsIP
Application Notes
Rabbit IgG F(ab')2 Agarose is suitable for immunoprecipitation and Immunoaffinity purifications.

Key Properties

SourceRabbit
Biological OriginRabbit
IsotypeIgG F(ab')2
ConjugationAgarose
PurityThis product is an purified Rabbit IgG isolated from normal Rabbit Serum digested by the enzyme pepsin and purified by chromatography coupled to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5 mg of anti-Rabbit IgG.

Storage & Handling

StorageStore vial at 4° C prior to opening. DO NOT FREEZE.
Form/AppearanceSuspension of agarose beads
Buffer/PreservativesPreservative: 0.5% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Rabbit IgG F(ab')2 fragment Agarose Conjugate, Rabbit IgG Fab2 Agarose

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Rabbit IgG F(ab')2 Agarose Antibody

SDS-PAGE of Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane 2: Reduced Rabbit IgG F(ab) Fragment (p/n orb2652745). Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n orb346310). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n orb346313). Load: 1 µg for F(ab) and F(c); 1.2 µg for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
IP
Immunoprecipitation
View Protocol

Rabbit IgG F(ab')2 Agarose Antibody (orb346308)

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