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| Catalog Number | orb782663 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Erythropoietin(EPO). |
| Tested applications | ELISA |
| Reactivity | Rabbit |
| Range | 46.88-3000 pg/mL |
| Assay Time | 3.5h |
| Concentration | 3000 pg/mL |
| UniProt ID | Q9GKA2 |
| Sensitivity | 17.7 pg/mL |
| Alternative names | EP ELISA Kit, Epoetin ELISA Kit, Erythropoetin ELI Read more... |
| Note | For research use only |
| Application notes | standard: 3000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit EPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit EPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit EPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit EPO in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |
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