Rabbit Complement C5 Protein

• Catalog Number: orb624214
• Category: Proteins
• Description: Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
• Reactivity: Rabbit
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 73.8 kDa (β chain) & 111.9 kDa (α chain)
• Target: Complement C5
• Source: Rabbit Complement C5, His Tag (orb624214) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Ala 1678 (Accession # XP_008271599.1).
• Expression System: HEK293
• Expression Region: Gln 19 - Ala 1678
• Endotoxins: 1.0 EU per μg
• NCBI: XP_008271599.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: Complement C5,C5,CPAMD4
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. During cultivation, Complement C5 will be partially processed into α and β chain and has a calculated MW of 73.8 kDa (β chain) and 111.9 kDa (α chain) respectively. The protein migrates as 80 kDa (β chain), 120 kDa (α chain) and 200 kDa (α & β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.