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Catalog Number | orb782850 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cr(Creatinine) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cr(Creatinine). |
Tested applications | ELISA |
Reactivity | Rabbit |
Range | 1.25-80 nmol/mL |
Assay Time | 2.5h |
Concentration | 80 nmol/mL |
Sensitivity | 0.74 nmol/mL |
Alternative names | CR ELISA Kit, Calretinin ELISA Kit, CALB2 ELISA Ki Read more... |
Note | For research use only |
Application notes | standard: 80 nmol/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rabbit CR protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit CR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit CR in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Expiration Date | Please enquire. |