Rabbit UCH-L3 Antibody

SKU: orb1525713

Description

UCH-L3 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Porcine, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 180 and C-terminus
• Target: UCH-L3
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

testicular tissue protein Li 221; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L3; ubiquitin thioesterase L3; ubiquitin thiolesterase; UCH-L3

Detection of human and mouse UCH-L3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-UCH-L3 antibody orb1525713 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. UCH-L3 was also immunoprecipitated by rabbit anti-UCH-L3 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Detection of human UCH-L3 by immunohistochemistry.Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-UCH-L3 (Cat. No. orb1525713) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

UCH-L3

UniProt

P15374

RefSeq (Protein):NP_005993.1