Rabbit TBP1 Antibody

SKU: orb1524643

Description

TBP1 Antibody

Research Area

Immunology & Inflammation, Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat, Xenopus
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 389 and C-terminus
• Target: TBP1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

26S protease regulatory subunit 6A; 26S proteasome AAA-ATPase subunit RPT5; 26S proteasome regulatory subunit 6A; human immunodeficiency virus tat transactivator binding protein-1; proteasome (prosome, macropain) 26S subunit, ATPase, 3; Proteasome 26S subunit ATPase 3; proteasome subunit P50; Tat-binding protein 1; TBP1; TBP-1; testicular secretory protein Li 42

Detection of human TBP1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, MCF-7, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TBP1 antibody (orb1524643) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of mouse TBP1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TBP1 antibody (orb1524643) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human TBP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBP1 antibody (orb1524643) used for IP at 6 µg per reaction. TBP1 was also immunoprecipitated by a previous lot of this antibody (orb1524643) and a second antibody against a different epitope of TBP1.

Detection of human TBP1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TBP1 (orb1524643) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

TBP1

UniProt

P17980

RefSeq (Protein):NP_002795.2