Rabbit RNF2 Antibody

SKU: orb1525845

Description

RNF2 Antibody

Images & Validation

• Tested Applications: ChIP, IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 175 and 225
• Target: RNF2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BAP1; BAP-1; DING; E3 ubiquitin-protein ligase RING2; HIP2-interacting protein 3; HIPI3; huntingtin-interacting protein 2-interacting protein 3; protein DinG; RING finger protein 1B; RING finger protein 2; RING finger protein BAP-1; RING1B; RING2; RING-type E3 ubiquitin transferase RING2

Detection of human and mouse RNF2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from K-562 (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RNF2 antibody orb1525845 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. RNF2 was also immunoprecipitated by rabbit anti-RNF2 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Detection of human RNF2 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RNF2 (Cat. No. orb1525845) used at a dilution of 1: 1, 000 (1µg/ml). Detection: Vector ImmPACT NovaRED

Quick Database Links

Gene Symbol

RNF2

UniProt

Q99496

RefSeq (Protein):NP_009143.1