Rabbit REDD1 Antibody

SKU: orb1527090

Description

REDD1 Antibody

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: ICC, IP, WB
• Dilution Range: WB - 1:1,000 - 1:4,000; IP - 5 - 15 µg/mg lysate; ICC-IF - 1:500 - 1:2,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Monkey, Mouse, Rabbit, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1 and 50
• Target: REDD1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dig2; DNA damage-inducible transcript 4 protein; HIF-1 responsive protein RTP801; protein regulated in development and DNA damage response 1; REDD1; REDD-1

Detection of human REDD1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Lysate was prepared from cells that had been treated with cobalt chloride (A and B) or mock treated (A). Antibodies: Affinity purified rabbit anti-REDD1 antibody orb1527090 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. REDD1 was also immunoprecipitated, albeit poorly, by rabbit anti-REDD1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Detection of human REDD1 by immunocytochemistry.Sample: NBF-fixed asynchronous, Cobalt-treated HeLa cells. Antibody: Affinity purified rabbit anti-REDD1 (Cat. No. orb1527090) used at a dilution of 1: 500 (2 µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG H&L cross-adsorbed Antibody DyLight®594 used at a dilution of 1: 100.

Quick Database Links

Gene Symbol

REDD1

UniProt

Q9NX09

RefSeq (Protein):NP_061931.1