DDIT4 Rabbit Polyclonal Antibody

• Catalog Number: orb4565
• Category: Antibodies
• Description: DDIT4 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Equine, Human, Mouse, Porcine, Rabbit
• Reactivity: Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DDIT4 (165-232/232aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 25 kDa
• Target: DDIT4
• UniProt ID: Q9NX09
• RRID: AB_10954828
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: DDIT4; DDIT4_HUMAN; Dig2; DKFZp564O2071; DNA damag
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Alireza Sadeghipour # 1 2, Fahimeh Fattahi # 2 3 4, Zahra Madjd 2 3, Fatemeh Tajik 2 5, Farnoosh Sedaghati # 6, Leili Saeednejad Zanjani Increased nuclear expression of DNA damage inducible transcript 4 can serve as a potential prognostic biomarker in patients with gliomas: a study based on data mining and experimental tools Discov Oncol ., (2025)

PubMed: 39915428

Paraformaldehyde-fixed, paraffin embedded (rat heart tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DDIT4) Polyclonal Antibody, Unconjugated (orb4565) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.