Rabbit PKM2 Antibody

SKU: orb1524429

Description

PKM2 Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Metabolism Research

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1 and 50
• Target: PKM2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CTHBP; cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; HEL-S-30; OIP3; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; PK3; PKM2; pyruvate kinase 2/3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; pyruvate kinase PKM; pyruvate kinase, muscle; TCB; THBP1; thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tumor M2-PK

Detection of human PKM2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PKM2 antibody orb1524429 used for WB at 0.1 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. PKM2 was also immunoprecipitated by rabbit anti-PKM2 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).

Detection of human PKM2 by immunohistochemistry. Samples: FFPE sections of human stomach. Antibody: Affinity purified rabbit anti-PKM2 antibodies. Detection: DAB

Detection of human PKM2 by immunohistochemistry. Samples: FFPE sections of human osteosarcoma. Antibody: Affinity purified rabbit anti-PKM2 antibodies. Detection: DAB

Quick Database Links

Gene Symbol

PKM2

UniProt

P14618

RefSeq (Protein):NP_002645.3