PKM2 Antibody

• Catalog Number: orb395450
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PKM2-specific.
• Target: PKM2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Immunogen: PKM2 pyruvate kinase, muscle
• UniProt ID: P14618
• Tested applications: ELISA, IF, IHC, IP, WB
• Dilution range: WB: 1:1000-1:4000; IP: 1:200-1:1000; IHC: 1:100-1:400; IF: 1:10-1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

MCF7 cells were subjected to SDS PAGE followed by western blot with orb395450 (PKM2-specific antibody) at dilution of 1:1500.

Immunofluorescent analysis of MCF-7 cells, using orb395450 (PKM2-specific Antibody) at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-PKM2-specific (IP: orb395450, 4 ug; Detection: orb395450 1:300) with MCF-7 cells lysate 3250ug.

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using orb395450 (PKM2-specific Antibody) at dilution of 1:200.