Rabbit Goat IgG (H&L) Antibody (Alkaline Phosphatase)

SKU: orb1236948

Description

Rabbit Goat IgG (H&L) Antibody (Alkaline Phosphatase)

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Goat
• Application Notes:
  Alkaline Phosphatase Conjugated Affinity Purified anti-Goat IgG . is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:1,000 - 1:4,000, Western Blot 1:500 - 1:2,000, Immunohistochemistry 1:200 - 1:1,000. (Optimal dilution should be determined by user).

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Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Isotype: IgG
• Immunogen: Goat IgG - whole molecule
• Purification: Alkaline Phosphatase Conjugated Affinity Purified anti-Goat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.
• Conjugation: AP

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.01% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (rAlbumin)
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only