Rabbit ERF Antibody

SKU: orb1527943

Description

ERF Antibody

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended. orb1527943 has not performed satisfactorily when used for WB of ERF in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ERF.; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 498 and C-terminus
• Target: ERF
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CHYTS; CRS4; ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE2; PE-2

Detection of human ERF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ERF antibody orb1527943 used for IP at 3 µg/mg lysate.

Detection of human and mouse ERF by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-ERF (Cat. No. orb1527943) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

ERF

UniProt

P50548

RefSeq (Protein):NP_006485.2