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Rabbit Bub3 Antibody
Description
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB - 1:5,000 - 1:15,000; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 278 and C-terminus |
| Target | Bub3 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human Bub3 by western blot.Samples: Whole cell lysate from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Bub3 antibody orb1530301 at 0.066 µg/ml.

Detection of human Bub3 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Bub3 used at a dilution of 1:1, 000 (1µg/ml).
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_004716.1 |
|---|
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Protocol Information
Rabbit Bub3 Antibody (orb1530301)
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