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Rabbit AKT1 Antibody
SKU: orb1527296
Description
Research Area
Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Metabolism Research, Neuroscience
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 25 and 75 |
| Target | AKT1 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−AKT; AKT1m; CWS6; PKB; PKB alpha; PKB-ALPHA; PRKBA; Protein kinase B; protein kinase B alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
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Detection of human AKT1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T (T) , MCF-7 (M) , Jurkat (J) and PANC1 (P) cells. Antibody: Affinity purified rabbit anti-AKT1 antibody orb1527296 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human AKT1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-AKT1 (Cat. No. orb1527296) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_005154.2 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
Rabbit AKT1 Antibody (orb1527296)
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