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| Catalog Number | orb2667919 |
|---|---|
| Category | Assays and Kits |
| Description | Rabbit Adiponectin (ADP) ELISA Kit |
| Application notes | standard: 500 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit ADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit ADP in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 7.82-500 ng/mL |
| Sensitivity | 4.75 ng/mL |
| Sample Types | serum, plasma and other biological fluids |
| Alternative names | GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And C Read more... |
| Note | For research use only |
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