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qPCR ProbesMaster Lyophilisate
Catalog Number: orb1733722
| Catalog Number | orb1733722 |
|---|---|
| Category | Small Molecules |
| Description | qPCR ProbesMaster Lyophilisate is designed for quantitative real-time analysis of DNA samples using DNA probe based detection. The lyophilisate is recommended for use with Dual Labeled Fluorescent Probes, e.g. TaqMan, Molecular Beacons or FRET probes. It provides an easy-to-handle and powerful tool for quantification of sample DNA in a broad dynamic range of up to 6 orders of magnitude with exceptional sensitivity and precision. The lyophilisate contains all reagents required for qPCR (except template, primer and labeled fluorescent probe) in a single bead. The high specificity and sensitivity of the mix based on an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup. |
| Storage | store at ambient temperature. Store in an aluminium-coated bag or on a dry place. Lyophilisates may hydrate at humidity levels >70 % when sealing is opened |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
For example, if the dosage of your experiment is 10mg/kg, the weight of each animal is 50g, and the volume of the dosing is 100μL, then the working solution concentration of your experiment is 5mg/mL. If the formula you used is 5% DMSO+ 30% PEG300+ 5% Tween 80 + 60% Saline/PBS/ddH2O, the preparation method is as follows:
Mother liquor preparation method: 5mg of drug dissolved in 100μL DMSO (mother liquor concentration is 50mg/mL). Preparation method of in vivo formulation: For 1mL of working solution preparation, take 50μL of DMSO mother liquor, add 300 μL of PEG300 to mix well and clarify, then add 50μL of Tween-80 to mix well and clarify, and finally add 600μL of Saline/PBS/ddH2O to mix well and clarify.Note:
- For Reference Only. Please choose the appropriate dissolution protocol according to your experimental animal and administration method.
- We suggest that in cell experiments, the final concentration of DMSO is not more than 0.1%, in animal experiments, for healthy mice, the final concentration of DMSO is not more than 10%; For nude or frail mice, the final concentration of DMSO is not more than 2%.
