Pyrrolidinedithiocarbamate ammonium

SKU: orb1304492

Description

This small molecule inhibitor selectively targets NF-κB to block the translation of nitric oxide synthase (NOS) mRNA, thereby preventing its induction. It is widely used in research to study inflammation, oxidative stress, and cell signaling pathways in both in vitro and in vivo experimental models.

Research Area

Signal Transduction

Key Properties

• CAS Number: 5108-96-3
• MW: 164.29
• Purity: >99.99% (May vary between batches)
• Formula: C₅H₁₂N₂S₂
• SMILES: [NH4+].[S-]C(=S)N1CCCC1
• Target: NF-κB
• Solubility: DMSO:247.5 mg/mL (1506.48 mM);5% DMSO+95% Saline:0.6 mg/mL (3.65 mM)

Bioactivity

• In Vivo:
  The DSS+PDTC-treated groupⅡ exhibited suppression of shortening of intestinal length and reduction of DAI score. Activated NF-κB level and IL-1β and TNF-α levels are significantly lower in DSS+PDTC-treated groupⅡ. These findings suggest that suppression of NF-κB activity by PDTC can delay the healing of mucosal tissue defects (erosions or ulcers) arising from inflammation, but that it can strongly suppress the expression of inf-lammatory cytokines (IL-1β and TNF-α), resulting in significant alleviation of colitis. PDTC is useful for the treatment of ulcerative colitis.
• In Vitro:
  Pretreatment of cells with PDTC (3-1000 mM) dose-dependently attenuate IL-8 production. Furthermore, PDTC (100 mM) suppresses the accumulation of IL-8 mRNA. PDTC inhibites the activation of NF-kB, because PDTC suppresses both NF-kB DNA binding and NF-kB-dependent transcriptional activity. Taken together, our data demonstrate that NF-kB inhibition with PDTC decrease IL-8 production by intestinal epithelial cells.
• Cell Research:
  The human colon cancer cell line HT-29 is obtained and cells are grown in modified McCoy's 5A medium supplemented with 10% fetal bovine serum. To study the effect of PDTC on IL-8 production, HT-29 cells in 96-well plates are induced with 20 ng/mL of IL-1b for 18 h. Various concentrations (3-1000 mM) of PDTC or its vehicle (culture medium) are added to the cells 30 min prior to IL-1b stimulation. The concentration of IL-8 in the supernatant is determined using solid-phase enzyme-linked immunosorbent assay, as described previously employing the multiple antibody sandwich principle that specifically detects human IL-8.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

colitis, Ammonium pyrrolidinedithiocarbamate, APDC, 1-Pyrrolidinedithiocarboxylic acid ammonium, 1-Pyrrolidinedithiocarboxylic acid ammonium salt, NFkB, NF-kB, NFκB, NF-κB, PDTC, PDTC ammonium, Pyrrolidinedithiocarbamate ammonium, ulcerative

Compound Identifiers

PubChem CID

517348