Pyraclostrobin

SKU: orb1940944

Description

Pyraclostrobin is a strobilurin fungicide that inhibits mitochondrial complex III of fungal and mammalian cells. Pyraclostrobin induces triglyceride accumulation and triglyceride accumulation in 3T3-L1 cells.

Key Properties

• CAS Number: 175013-18-0
• MW: 387.82
• Purity: >98% (HPLC)
• Formula: C??H??ClN?O?
• SMILES: COC(N(C1=C(C=CC=C1)COC2=NN(C3=CC=C(Cl)C=C3)C=C2)OC)=O
• Target: Fungal
• Solubility: DMSO : 250 mg/mL (644.63 mM; Need ultrasonic)

Bioactivity

• In Vivo:
  Animal model: Zebrafish. Dosage: 0.001 mg/L, 0.01 mg/L, 0.02 mg/L. Administration: Fish were assigned to a vessel containing 20 L of water dissolved Pyraclostrobin. Result: Showed higher ROS levels in zebrafish livers. Decreased SOD levels and increased MDA levels. Increased the olive tail moments as the dose increased on days 7, 14, 21, and 28. Animal model: Zebrafish larvae. Dosage: 33 mg/L, 36 mg/L, 40 mg/L, 44 mg/L, 48 mg/L. Administration: Zebrafish larvae at 4 days post fertilization (dpf) were transferred into 24-well plates and subjected to pyraclostrobin until 8 dpf. Result: Damaged histological and subcellular structure of larval heart and brain. Changed the expression level of cardiac muscle contraction pathway-and neural-related genes and proteins. Impaired larval cardiac function and locomotor behavior.
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• In Vitro:
  Cell Viability Assay Cell line: HepG2 cells. Concentration: 10 μmol/L, 20 μmol/L, 40 μmol/L, 80 μmol/L. Incubation time: 24 h. Result: Inhibited cell survival in concentration-dependent manner with IC50 value of 30.22 μmol/L. Western blot analysis. Cell line: HepG2 cells. Concentration: 10 μmol/L, 20 μmol/L, 40 μmol/L, 80 μmol/L. Incubation time: 6 h. Result: Increased the content of cytochrome c (Cyt c) in the cytoplasm in a concentration-dependent way. Increased pro-apoptotic protein Bax expression and down-regulated anti-apoptosis protein Bcl-2 expression.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Fungal