PYCARD Antibody

SKU: orb1247908

Description

PYCARD Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 19kDa band observed in lysates of cell line HeLa (calculated MW of 20.0kDa according to NP_660183.1). Recommended concentration: 0.2-0.6µg/mL. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Liver shows staining of sinusoidal lining cells. Recommended concentration: 4-6µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RESQSYLVEDLERS
• Target: PYCARD
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PYCARD, ASC, TMS1, PYD and CARD domain containing, CARD5, MGC10332, TMS, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain protein 5, target of methylation-induced silencing-1, TMS-1

orb1247908 (0.2 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247908 (4 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Quick Database Links

Gene Symbol

PYCARD

UniProt

Q9ULZ3

RefSeq (Protein):NP_660183.1, NP_037390.2