Puromycin dihydrochloride

SKU: orb1306074

Description

Puromycin dihydrochloride

Research Area

Cell Biology, Infectious Disease & Virology, Molecular Biology

Key Properties

• CAS Number: 58-58-2
• MW: 544.44
• Purity: 99.89%
• Formula: C₂₂H₃₁Cl₂N₇O₅
• SMILES: Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@@H]2[C@@H](CO)O[C@H]([C@@H]2O)n2cnc3c(ncnc23)N(C)C)cc1
• Target: ribosome,Antibiotic,Parasite,DNA/RNA Synthesis,Antibacterial
• Solubility: H2O:92 mg/mL (168.98 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:255 mg/mL (468.37 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (9.18 mM)

Bioactivity

• Target IC50:
  MOLT-4 cells:0.17 μM (EC50)|KB-3-1 cells:0.21 μM|PRH cells:2000 nM|HEK293 cells:0.35 nM|J774.A1 cells:10 μM|HEK293T cells:0.42 μM (CC50)|K562 cells:0.22 μM|A549 cells:300 nM|HepG2 cells:1600 μM|KB-V1 cells:79.3 μM
• In Vivo:
  Puromycin is utilized to select recombinant cells from non-cultured cells. It serves as a tool for studying protein synthesis in many systems.
• In Vitro:
  METHODS: Human hepatocellular carcinoma cells, HepG2, and primary rat hepatocytes, PRH, were treated with Puromycin dihydrochloride (0-300 µmol/L) for 72 h, and cell growth inhibition was detected by MTT. RESULTS: Puromycin dihydrochloride dose-dependently inhibited the growth of HepG2 and PRH cells, with IC50s of 1600 μmol/L and 2000 nmol/L, respectively. METHODS: Human breast cancer cells MDA-MB-231 and MDA-MB-436 were transfected with shRNAs-Puro lentivirus, and after 24 h, the transfected cells were cultured in new medium containing Puromycin dihydrochloride (1 μg/ml) for about 12 days, and then the cells were screened for the success of transfection. RESULTS: Puromycin dihydrochloride screened the lentivirally transfected cells successfully. METHODS: Cells transfected with lentivirus were cultured in medium supplemented with Puromycin dihydrochloride (2 μg/mL). RESULTS: Puromycin dihydrochloride screened and maintained lentivirally successfully transfected cells.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CL-13900, CL13900 dihydrochloride, CL13900 Dihydrochloride, CL-13900 Dihydrochloride, CL 13900, CL 13900 Dihydrochloride, CL13900, DNASynthesis, DNA Synthesis, DNA synthesis, 60S ribosome, Bacterial, Antibiotic, inhibit, Inhibitor, Puromycin, Puromycin 2HCl, Puromycin Dihydrochloride, Puromycin dihydrochloride, RNASynthesis, RNA Synthesis

Compound Identifiers

PubChem CID

443311