Purified recombinant Green Fluorescent Protein (wild type)

• Catalog Number: orb1972372
• Category: Proteins
• Description: Purified recombinant Green Fluorescent Protein (wild type)
• Form/Appearance: Each vial contains 100µg 1mg/ml purified recombinant GFP (wild type) in 10 mM TRIS buffer pH 8.0, containing 0.02% sodium azide.
• Concentration: 1mg/ml
• UniProt ID: P42212
• Tested applications: ELISA, WB
• Source: Wild type recombinant GFP (from A.victoria) expressed in E.coli and purified by high resolution gel filtration.
• Storage: The antibody may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

The purity of the recombinant GFP (wild type) protein is >98%, as confirmed by SDS-polyacrylamide gel electrophoresis, specific absorbance peaks, HPLC and A397/280 ratio.