Puerarin 6''-O-Xyloside

SKU: orb1301258

Description

Puerarin 6''-O-xyloside is a bioactive small molecule with demonstrated anti-osteoporotic effects in ovariectomized mouse models and antitumor activities. Its mechanisms involve the modulation of key apoptotic proteins, including the upregulation of caspase-3, -7, -9, and Bax alongside the downregulation of Bcl-2, supporting its use in bone metabolism and oncology research.

Research Area

Cell Biology, Protein Biochemistry

Key Properties

• CAS Number: 114240-18-5
• MW: 548.49
• Purity: 98.67% (May vary between batches)
• Formula: C₂₆H₂₈O₁₃
• SMILES: O[C@@H]1CO[C@@H](OC[C@H]2O[C@H]([C@H](O)[C@@H](O)[C@@H]2O)c2c(O)ccc3c2occ(-c2ccc(O)cc2)c3=O)[C@H](O)[C@H]1O
• Target: Apoptosis,Caspase
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.65 mM);DMSO:25 mg/mL (45.58 mM)

Bioactivity

• In Vivo:
  The in vivo animal study showed that POS significantly inhibited tumour growth in A549 cells (p < 0.01). The POS has significant antitumour activities. The mechanisms are related to increased levels of caspase-3, caspase-7, caspase-9 and Bax, and reduced levels Bcl-2.
• Animal Research:
  The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cytotoxicity of POS (at 10, 20 and 40 μM) in vitro, and xenograft nude mice were established to evaluate the antitumour effect of POS (at 40 mg/kg/d) in vivo by 15 days intraperitoneal injection (ip). To explore its mechanism of action, flow cytometry was performed to determine the pro-apoptotic effect of POS (at 10, 20 and 40 μM). Subsequently, the expression of caspase-3, caspase-7, caspase-9, Bcl-2 and Bax in A549 cells were determined.

Storage & Handling

• Storage: keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Puerarin 6'' O Xyloside, Puerarin 6''OXyloside, Puerarin 6''-O-Xyloside, Apoptosis, Caspase, Inhibitor, inhibit

Compound Identifiers

PubChem CID

100990912