Proteinase K - Solution

• Catalog Number: orb532707
• Category: Tools
• Description: Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The Protein with a molecular weight of 28.9 kDa cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinse K is not inactivated by metal chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12.5). Proteinase K is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF. In presence of 0.5-1% SDS Proteinase K inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.
• CAS Number: 39450-01-6
• Concentration: 20 mg/ml
• Form/Appearance: Proteinase K solution in 10 mM Tris-HCl, 1 mM Ca(H 3C2O2)2, 10 % (v/v) Glycerol, pH 7.8 (22°C)
• Purity: free of RNases, DNases and Exonucleases
• MW: Theoretical MW: 28.9 kDa
• Biological Activity: > 600 mAnsonU/ml
• Storage: store at -20 °C
• Note: For research use only
• Application notes: Digestion of proteins during DNA and RNA preparation.
• Expiration Date: 6 months from date of receipt.