Protease S. aureus

• Catalog Number: orb420132
• Category: Proteins
• Description: Protease S. aureus
• Concentration: 1 mg/mL
• Form/Appearance: Lyophilized
• Purity: Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay.
• Conjugation: Unconjugated
• Target: sspA
• Hazard Information: Non-Toxic
• UniProt ID: P0C1U8
• Biological Activity: 1,980 units/mg by dry weight
• NCBI: 1280
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: Preservative: None. Stabilizer: None. None
• Alternative names: Glutamyl endopeptidase, Endoproteinase Glu-C, Stap
• Note: For research use only
• Application notes: Protease S. aureus has shown that one unit causes a change of 0.001 A280 nm per minute at 37° C at pH 7.8 using casein as a substrate. Specific conditions for reactivity should be optimized by end user.
• Expiration Date: 12 months from date of receipt.