Progesterone Receptor Antibody

• Catalog Number: orb248505
• Category: Antibodies
• Description: This antibody is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of progesterone receptor has been suggested to reflect a intact estrogen regulatory machinery and therefore predict better clinical response to endocrine therapy than ER alone.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PR501
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human Progesterone Receptor protein was used as the immunogen for this antibody.
• Dilution range: Western blot: 0.5-1ug/ml for 2 hr at RT,Immunohistochemistry (FFPE): 0.5-1ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Progesterone receptor antibody is available for research use only.
• Entrez: 5241
• Storage: Store the Progesterone receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of formalin-fixed and paraffin-embedded normal human breast tissue using Progesterone R antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded invasive ductal carcinoma tissue using Progesterone R antibody