Proflavine Hemisulfate

SKU: orb1225884

Description

Proflavine Hemisulfate is a topical antiseptic by interchelating DNA, thereby disrupting DNA synthesis and leading to high levels of mutation in the copied DNA strands.(In Vitro):Proflavine (0.1-10 μM; 24 hours) inhibits the growth of Kir3.2-transformant cells and Kir3.2 activity in a concentration-dependent manner.Proflavine (300 μM) progressively reduces the current amplitude of Kir3.2 mutant to 27.7±4.3% of the control.(In Vivo):Proflavine (0.1-10 μM; 24 hours) inhibits the growth of Kir3.2-transformant cells and Kir3.2 activity in a concentration-dependent manner.Proflavine (300 μM) progressively reduces the current amplitude of Kir3.2 mutant to 27.7±4.3% of the control.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 1811-28-5
• MW: 516.57
• Purity: >98% (HPLC)
• Formula: C₂₆H₂₄N₆₀₄S
• SMILES: C1=CC(=CC2=NC3=C(C=CC(=C3)[NH3+])C=C21)N.C1=CC(=CC2=NC3=C(C=CC(=C3)[NH3+])C=C21)N.[O-]S(=O)(=O)[O-]
• Target: DNA/RNA Synthesis
• Solubility: Water: 22 mg/mL (85.18 mM)

Bioactivity

• In Vivo:
  The concentrations of Proflavine (20 mg/kg) in whole blood after intravenous injection decreased rapidly at the beginning and remained stable from around 30 min after dosing. Animal model: Adult male Sprague Dawley rats (weighing approximately 200 g). Dosage: 20 mg/kg (Pharmacokinetic Analysis). Administration: Intravenous injection; 2, 4, 5, 10, 15, 20, 25, and 30 min after dosing. Result: Concentration decreased rapidly from whole blood in the first 5 min after dosing, followed by a slower decrease.
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• In Vitro:
  Proflavine (0.1-10 μM; 24 hours) inhibits the growth of Kir3.2-transformant cells and Kir3.2 activity in a concentration-dependent manner. Proflavine (300 μM) progressively reduces the current amplitude of Kir3.2 mutant to 27.7±4.3% of the control. Cell Viability Assay Cell line: Kir3.2*-transformant BYT123 cells Concentration: 0.1, 1, and 10 μM Incubation time: 24 hours. Result: Dose-dependent inhibition of the growth of Kir3.2*-transformant cells. Attenuated the growth of Kir3.2*-transformant cells without affecting the growth of control cells.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Proflavine hemisulfate | Proflavine hemisulphate | EINECS 217-320-3

Quick Database Links

Gene Symbol

DNA/RNA Synthesis