Procyanidin B1

SKU: orb1296133

Description

Procyanidin B1 is a natural polyphenol derived from common fruits that acts as a TLR4/MD-2 complex antagonist. This mechanism underlies its anti-inflammatory properties, making it a useful research tool for studying inflammation in both cellular and animal models of immune-related diseases.

Research Area

Immunology & Inflammation

Key Properties

• CAS Number: 20315-25-7
• MW: 578.52
• Purity: 99.91% (May vary between batches)
• Formula: C₃₀H₂₆O₁₂
• SMILES: O[C@H]1Cc2c(O)cc(O)c([C@@H]3[C@@H](O)[C@H](Oc4cc(O)cc(O)c34)c3ccc(O)c(O)c3)c2O[C@@H]1c1ccc(O)c(O)c1
• Target: TLR
• Solubility: DMSO:23 mg/mL (39.76 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.46 mM)

Bioactivity

• In Vitro:
  Procyanidin B1 (100 μg/mL) inhibits LPS-induced TNF-α production, and expression of MD-2, TRAF6, NF-κB mRNA, phosphorylated p38 MAPK and NF-κB protein in THP1 cells . Procyanidin B1 (50-100 μM) protects against Aβ oligomer-induced neuronal death. Procyanidin B1 potently inhibits the activation of caspase-3 at 100 μM, caspase-8 at concentrations of 30, 50, and 100 μM and caspase-9 at concentrations of 10, 30, 50, and 100 μM . Procyanidin B1 (10, 20, 30 μM) significantly and dose-dependently induces expression of ACO and CPT1, with no obvious effect on mRNA expression of PPARα .
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• Cell Research:
  To investigate the cytotoxic effect of Procyanidin B1, the viability of THP1 cells is assessed using the CCK8 assay. THP1 cells are treated with Procyanidin B1 for 18 h, and 10 μL of CCK8 solution is then added to each well and the cultures are incubated for 4 h at 37°C. The optical density (OD) at 450 nm is measured using an ELx808 Absorbance Microplate Reader. The Procyanidin B1 concentration tested ranges from 50 to 200 μg/mL. Each sample is tested in triplicate .
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, MD2-TLR4, Inhibitor, TLR4/MD-2, TLR, Procyanidin B1, Procyanidin B-1, Procyanidin B 1, Toll-like Receptor (TLR)

Compound Identifiers

PubChem CID

11250133