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Catalog Number | orb1259534 |
---|---|
Category | Antibodies |
Description | PRG4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1264-1363 of human PRG4 (NP_001121180.2). |
Concentration | batch dependent |
Dilution range | WB: 1:200 - 1:2000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 150kDa |
Target | PRG4 |
UniProt ID | Q92954 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | PRG4, proteoglycan 4, CACP, FLJ32635, HAPO, JCAP, Read more... |
Note | For research use only |
Application notes | WB: 1:200 - 1:2000 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of LO2 cells, using PRG4 antibody (orb1259534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
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