Human Proteoglycan 4 (PRG4) ELISA Kit

SKU: orb777292

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteoglycan 4(PRG4).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRG4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRG4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRG4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRG4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, synovial fluid and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 62.5-4000 pg/mL
• Sensitivity: 24.6 pg/mL
• Concentration: 4000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MSF ELISA Kit, CACP ELISA Kit, HAPO ELISA Kit, JCAP ELISA Kit, SZP ELISA Kit, Megakaryocyte Stimulating factor ELISA Kit, Articular Superficial Zone Protein ELISA Kit, Camptodactyly ELISA Kit, Arthropathy,Coxa Vara,Pericarditis Syndrome ELISA Kit

Quick Database Links

UniProt

Q92954