Pregnenolone Carbonitrile

SKU: orb1306187

Description

Pregnenolone 16α-carbonitrile (PCN) is a well-characterized, potent agonist of the rodent pregnane X receptor (PXR). Its primary research application is as a model inducer of CYP3A enzyme expression, facilitating studies of drug metabolism and nuclear receptor signaling in both in vitro and in vivo experimental systems.

Research Area

Metabolism Research

Key Properties

• CAS Number: 1434-54-4
• MW: 341.49
• Purity: 99.69% (May vary between batches)
• Formula: C₂₂H₃₁NO₂
• SMILES: [H][C@@]12C[C@@H](C#N)[C@H](C(C)=O)[C@@]1(C)CC[C@@]1([H])[C@@]2([H])CC=C2C[C@@H](O)CC[C@]12C
• Target: Cytochromes P450
• Solubility: DMSO:30 mg/mL (87.85 mM)

Bioactivity

• In Vivo:
  In WT mice, Pregnenolone Carbonitrile (40 mg/kg/day; i.p.) induces the expression of CYP3A11 and CYP2B10 at the mRNA, protein, and enzymatic levels. In adult female Sprague-Dawley rats, Pregnenolone Carbonitrile (100 mg/kg; i.p.) induces the expression of CYP3A mRNA. In male Sprague-Dawley rats, Pregnenolone Carbonitrile (35 mg/kg; gavage) increase in Pgp expression.
• In Vitro:
  Pregnenolone Carbonitrile is hepatoprotective by increasing resistance to subsequent stressful insults.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pregnenolone Carbonitrile, Pregnenolone 16a-carbonitrile, Pregnenolone 16alpha-carbonitrile, Pregnenolone 16α-carbonitrile, Pregnenolone 16α-carbonitrile , Inhibitor, inhibit, orally, PXR, P4503A, cytochrome, Cytochrome P450, CYPs, CYP3A, 5-Pregnen-3beta-ol-20-one-16alpha-carbonitrile, 5-Pregnen-3b-ol-20-one-16a-carbonitrile, 5-Pregnen-3β-ol-20-one-16α-carbonitrile

Compound Identifiers

PubChem CID

15032