Prednisolone hemisuccinate

SKU: orb1707864

Description

Prednisolone hemisuccinate is a water-soluble prodrug of prednisolone, a potent glucocorticoid receptor agonist. It is widely used in research to study anti-inflammatory and immunosuppressive mechanisms in models of autoimmune diseases like lupus and arthritis, both in vitro and in vivo.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 2920-86-7
• MW: 460.52
• Purity: 99.96% (May vary between batches)
• Formula: C₂₅H₃₂O₈
• SMILES: C[C@]12C[C@H](O)[C@H]3[C@@H](CCC4=CC(=O)C=C[C@]34C)[C@@H]1CC[C@]2(O)C(=O)COC(=O)CCC(O)=O
• Target: Glucocorticoid Receptor
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (8.69 mM);DMSO:150 mg/mL (325.72 mM)

Bioactivity

• In Vitro:
  Methods: Peripheral blood mononuclear cells containing NK cells were isolated and cocultured with target K562 cells at three different effector-to-target (E:T) ratios of 50:1, 25:1, and 12.5:1. Prednisolone hemisuccinate or IVIG were then added to the tubes at an E:T ratio of 50:1 to assess the inhibitory effect. Percent killing was recorded and statistically analyzed using the Student t test. Results: In experiments with an E:T ratio of 50:1 and either Prednisolone hemisuccinate or IVIG in the coculture, the mean target cell killing rate was 26.4%. In cultures using the same E:T ratio, this killing rate was significantly reduced in the presence of IVIG (9.9%) or Prednisolone hemisuccinate (13.6%) (P < 0.001 for both analyses). Prednisolone hemisuccinate was able to inhibit the cytolytic activity of NK cells.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Prednisolone, Prednisolone 21-hemisuccinate, Prednisolone hemisuccinate

Compound Identifiers

PubChem CID

656804