PQ401

SKU: orb1705373

Description

PQ401

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 196868-63-0
• MW: 341.79
• Purity: 99.77%
• Formula: C₁₈H₁₆ClN₃O₂
• SMILES: COc1ccc(Cl)cc1NC(=O)Nc1cc(C)nc2ccccc12
• Target: Apoptosis,IGF-1R
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (2.93 mM);DMSO:17.1 mg/mL (50.03 mM);Ethanol:8.5 mg/mL (24.87 mM)

Bioactivity

• Target IC50:
  IGF-1R < μM
• In Vivo:
  Treated mice received 50 or 100 mg/kg PQ401 prepared in 8% polysorbate 80/ethanol/PBS, administered i.p. thrice a week.
• Cell Research:
  The inhibitory effects of diaryl urea on breast cancer cell growth are determined using a CyQuant cell proliferation assay kit. MCF-7 or MCNeuA cells are plated in 96-well plates (5 × 10^3 per well) in phenol red-free DMEM supplemented with 10% FCS. One plate isprepared for Each harvest day. Cells are allowed to adhere overnight and are then treated with various concentration of diaryl urea or DMSO as a Vehicle control. Microplate cultures are Harvested on days 0, 1, 2, and 3 by invertin the microplate onto paper towels with gentle blotting to remove growth medium without disrupting adherent cells. Each plate iskept at -80 ℃ unti the end o the experiment (day 3) when All o the plates are thawed and assayed together. After thawing, 200 μL of CyQuant GR solution are added to each well an the plates are incubated in the dark for 2 to 5 minutes. Fluorescence ismeasured with a SpectraMax Gemini XS fluorescence microplate reader with 480 nM excitation and 520 nM emission. Proliferation index iscalculated a the percent of nucleotide content versus control cells At day 0.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

cells, breast, caspase, cancer, autophosphorylation, Apoptosis, Inhibitor, IGF1R, IGF-1R, IGF-1R Inhibitor II, MCF-7, inhibit, PQ 401, PQ401, PQ-401

Compound Identifiers

PubChem CID

9549305