PPARA Antibody

SKU: orb305792

Description

Mouse monoclonal antibody to PPARA

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Metabolism Research

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF - 1:25, WB - 1:1000, IHC-P - 1:25, FC - 1:25
• Reactivity: Human, Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,κ
• Clone No.: 1331CT894.186.143
• Immunogen: Recombinant Protein
• Target: PPARA
• Molecular Weight: 52225
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Peroxisome proliferator-activated receptor alpha antibody, anti PPAR-alpha antibody, anti Nuclear receptor subfamily 1 group C member 1 antibody, anti PPARA antibody, anti NR1C1 antibody, anti PPAR antibody

Immunohistochemical analysis of paraffin-embedded H. kidney section using PPARA Antibody. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow cytometric analysis of Hela cells using PPARA Antibody (green) compared to an isotype control of mouse IgG1 (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using PPARA Antibody. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from Hela, Jurkat, mouse NIH/3T3 cell line (from left to right), using PPARA Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysates at 35 μg per lane.

Fluorescent image of Hela cells stained with PPARA Antibody. Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Quick Database Links

Gene Symbol

PPARA

UniProt

Q07869