PPARA Antibody

• Catalog Number: orb1927181
• Category: Antibodies
• Description: Purified Mouse Monoclonal Antibody (Mab)
• Target: This PPARA antibody is generated from a mouse immunized with a recombination protein from the human region of human PPARA.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,κ
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: Q07869
• MW: 52225 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:25, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25, FC: 1:25
• Clone Number: 1331CT894.186.143
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Peroxisome proliferator-activated receptor alpha,
• Note: For research use only

Flow cytometric analysis of Hela cells using PPARA Antibody (green) compared to an isotype control of mouse IgG1 (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded H. kidney section using PPARA Antibody. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using PPARA Antibody. Diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from Hela, Jurkat, mouse NIH/3T3 cell line (from left to right), using PPARA Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1: 3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Fluorescent image of Hela cells stained with PPARA Antibody. Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).