PPAR gamma phospho S273 Antibody

SKU: orb345808

Description

PPAR gamma (phospho-pS273) antibody

Images & Validation

• Tested Applications: DOT, ELISA, WB
• Dilution Range: ELISA: 1: 10,000, WB: 1ug/ml
• Reactivity: Human
• Application Notes:
  Anti-PPAR antibody has been tested by ELISA and dot blot and is useful for Immunohistochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~57kDa corresponding to the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-PPAR gamma pS273 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human PPARG protein.
• Purity: Anti-PPAR gamma pS273 was affinity purified from monospecific antiserum by immunoaffinity chromatography and is directed against the phosphorylated form of human PPAR gamma at the S273 residue. A BLAST analysis was used to suggest cross-reactivity with rat, mouse, primate, human, dog, bovine, and pig based on 100% sequence homology. Cross-reactivity with PPAR gamma pS273 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.05% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.85 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-PPAR gamma pS273 antibody, Peroxisome proliferator-activated receptor gamma, Nuclear receptor subfamily 1 group C member 3, NR1C3, PPARG, PPAR gamma

ELISA results of purified Rabbit anti-PPAR gamma pS273 tested against BSA-conjugated non-phospho and phospho forms of immunizing peptide. Each well was coated in duplicate with either 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n orb347654) and TMB substrate p/n orb348651.

Quick Database Links

UniProt

P37231

RefSeq:20336229