PPAR gamma Rabbit Polyclonal Antibody

SKU: orb11291

Description

PPAR gamma Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Gallus, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PPAR Gamma (101-200/505aa)
• Target: PPARG
• Molecular Weight: 57 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

GLM1; CIMT1; NR1C3; PPARG; PPARG1; PPARG2; PPARG5; PPARgamma; Nuclear receptor subfamily 1 group C member 3; PAX8/PPARG Fusion Gene; Peroxisome Proliferator Activated Receptor gamma; Peroxisome proliferator activated nuclear receptor gamma variant 1; Peroxisome proliferator activated receptor gamma 1; Peroxisome Proliferator Activated Receptor gamma; Peroxisome proliferator-activated receptor gamma; PPAR-gamma; PPARG_HUMAN; PPAR-γ; PPAR γ; PPARγ;

Blank control (blue line): U251, Primary Antibody (green line): Rabbit Anti-PPARG/PPAR gamma antibody (orb11291), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: A431. Primary Antibody (green line): Rabbit Anti-PPAR gamma antibody (orb11291), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PPAR gamma) Polyclonal Antibody, Unconjugated (orb11291) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Breast tissue lysates, Lane 2: Mouse Lung tissue lysates, Lane 3: Rat Adipose tissue lysates, Lane 4: Rat Breast tissue lysates, Lane 5: Rat Lung tissue lysates, Lane 6: Human A431 cell lysates, Lane 7: Human A549 cell lysates, Lane 8: Human THP-1 cell lysates, Lane 9: Human 293T cell lysates, Primary: Anti-PPAR gamma (orb11291) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kDa, Observed band size: 52 kDa.

Tissue/Cell: A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PPAR gamma) polyclonal Antibody, Unconjugated (orb11291) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: mouse lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PPAR Gamma Polyclonal Antibody, Unconjugated (orb11291) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

PPARG

UniProt

P37231

References

Ebrahim Elsangeedy * 1,4 , Dina N. Yamaleyeva * 1,4 , Nicholas P. Edenhoffer 3,4 , Allyson Deak 1,4 , Anna Soloshenko 1,4 , Jonathan Ray 1,4 , Xuming Sun 1,4 , Omar H. Shaltout 1,4 , Nildris Cruz Diaz 1,4 , Brian Westwood 1,4 , Daniel Kim-Shapiro 5 , Debra I. Diz 1,4 , Shay Soker 3,4 , Victor M. Pulgar 1,4,6 , April Ronca 4,7 , Jeffrey S. Willey 2,4 , and Liliya M. Yamaleyeva Sex-Specific Cardiovascular Adaptations to Simulated Microgravity in Sprague-Dawley Rats bioRxiv, (2024)

PubMed:

Applications

IHC

Reactivity

Rat

Shanhong Fang 1 2 3 4, Songye Wu 1 2 3 4, Peng Chen Targeting Caveolin-1 for enhanced rotator cuff repair: findings from single-cell RNA sequencing Cell Death Discov ., (2025)

PubMed: 40044676

Applications

WB

Reactivity

Human

Yang, Wucai et al. Melatonin promotes triacylglycerol accumulation via MT2 receptor during differentiation in bovine intramuscular preadipocytes Sci Rep, 7, 15080 (2017)

PubMed: 29118419