Mouse Peroxisome Proliferator Activated Receptor Gamma (PPAR-γ) ELISA Kit

• Catalog Number: orb775497
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Gamma(PPARg).
• Tested applications: ELISA
• Reactivity: Mouse
• Range: 0.16-10 ng/mL
• Assay Time: 3.5h
• Concentration: 10 ng/mL
• UniProt ID: P37238
• Sensitivity: 0.063 ng/mL
• Alternative names: PPAR-G ELISA Kit, PPARG1 ELISA Kit, PPARG2 ELISA K
• Note: For research use only
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PPAR-γ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PPAR-γ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PPAR-γ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PPAR-γ in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.

References

Runhong Zhou 1, Ruifeng Huang 2, Shaofen Zhou 2, Shengsheng Lu 2, Haixing Lin 2, Jingnan Qiu 2, Shuaiqi Ma 2, Jian He Sorbicillinoid HSL-2 inhibits the infection of influenza A virus via interaction with the PPAR-γ/NF-κB pathway J Infect Chemother, (2024)

PubMed: 38942291

Applications

ELISA

Reactivity

Mouse

Haiwei Ni # 1, Zachary J Reitman # 2, Wei Zou 1, Md Naushad Akhtar 1, Ritama Paul 1, Menggui Huang 1, Duo Zhang 1, Hao Zheng 1, Ruitao Zhang 1, Ruiying Ma 1, Gina Ngo 1, Lin Zhang 3, Eric S Diffenderfer 1, S Azar Oliaei Motlagh 1, Michele M Kim 1, Andy J Minn 1 4, Jay F Dorsey 1, Jessica B Foster 5, James Metz 1, Constantinos Koumenis 1 4, David G Kirsch 6 7 8 9, Yanqing Gong 10, Yi Fan FLASH radiation reprograms lipid metabolism and macrophage immunity and sensitizes medulloblastoma to CAR-T cell therapy Nat Cancer ., (2025)

PubMed: 39910249

Applications

ELISA

Reactivity

Mouse