Ponicidin

SKU: orb1304653

Description

Ponicidin (Rubescensine B) is a bioactive diterpenoid isolated from Rabdosia rubescens. It demonstrates immunoregulatory, anti-inflammatory, antiviral, and anticancer properties, such as inducing apoptosis in gastric carcinoma cells by inhibiting JAK2/STAT3 phosphorylation. Research applications include in vitro studies on cancer cell lines and in vivo models investigating its therapeutic potential.

Research Area

Cardiovascular Research, Cell Biology, Epigenetics & Chromatin, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 52617-37-5
• MW: 362.42
• Purity: 99.85% (May vary between batches)
• Formula: C₂₀H₂₆O₆
• SMILES: [H][C@]12CC[C@]3([H])[C@]4(C(=O)C1=C)[C@@]2([H])O[C@@]1([H])O[C@]4(O)[C@@H](O)[C@]2([H])C(C)(C)CC[C@H](O)C312
• Target: JAK|||STAT|||Apoptosis
• Solubility: DMSO:242.5 mg/mL (669.11 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (13.8 mM)

Bioactivity

• In Vitro:
  K562 cells in culture medium in vitro were given different concentrations of Ponicidin (10-50 micromol x L(-1)) for 24, 48, and 72 h. The inhibitory rate of the cells was measured by MTT assay, cell apoptotic rates were detected by flow cytometry (FCM) using Annexin V staining after K562 cells were treated with different concentrations of Ponicidin for 72 hours, and cell morphology was observed by Wright-Giemsa staining. Ponicidin (over 30 micromol x L(-1)) could inhibit the growth of K562 cells in both time- and dose-dependent manner. FCM analysis revealed that apoptotic cells were gradually increased in a dose-dependent manner after treatment for 72 hours, and that marked morphological changes of cell apoptosis such as condensation of chromatin was clearly observed by Wright-Giemsa staining after treatment by 50 micromol x L(-1) Ponicidin. Furthermore, Western blotting also showed that expression of p-AKT and p-P85 in PI3K/AKT signaling pathways was downregulated dramatically whereas the expression of p-P38, as well as p-ERK and p-JNK, remained unchanged after the cells were treated by PON for 48 h.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, JAK, JAK2, JNK, PARP, HSV, ERK, Akt, Apoptosis, Caspase, Bcl-2, Rubescensine B, ROS, STAT3, STAT, PI3K, Ponicidin

Compound Identifiers

PubChem CID

124223327