Polyinosinic-polycytidylic acid sodium

SKU: orb1218036

Description

Polyinosinic-polycytidylic acid sodium is a synthetic analog of double-stranded RNA and an agonist of toll-like receptor 3 (TLR3) and retinoic acid inducible gene I (RIG-I)-like receptors (RIG-I and MDA5).

Key Properties

• CAS Number: 42424-50-0
• MW: ——
• Purity: >98% (HPLC)
• Formula: (C₁₀H₁₃N₄O₈P)x.(C₉H₁₄N₃O₈P)x.xNa
• SMILES: C1=CN(C(=O)N=C1N)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)(O)O)O)O.C1=NC2=C(C(=O)N1)N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O)O)O)O.[Na]
• Target: TLR
• Solubility: H2O:10 mg/mL (Need ultrasonic and warming)

Bioactivity

• In Vitro:
  Cell Viability Assay Cell line: WM793, WM278, WM239A, WM9 and 1205Lu cells. Concentration: 20 ng/mL. Incubation time: 24 hours. Result: Strongly reduced viability from 100% in controls to 20%-50% within 24 hours. Apoptosis Analysis Cell line: 1205Lu cells. Concentration: 200 ng/mL. Incubation time: 24 hours. Result: Induced apoptosis in 1205Lu cells. RT-PCR Cell line: 1205Lu cells. Concentration: 3 ng/mL. Incubation time: 24 hours. Result: Induced IFN-β expression in melanoma cells. Western blot analysis. Cell line: 1205Lu cells. Concentration: 5 ng/mL. Incubation time: 24 hours. Result: Revealed active subunits of caspase-9 and caspase-8 in melanoma cells.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Poly(I:C) sodium

Quick Database Links

Gene Symbol

TLR