Poly(I:C)

SKU: orb1219267

Description

Poly (I:C) is a synthetic double-stranded RNA (dsRNA) analog and is an immunostimulant that acts as the most potent interferon (IFN) inducer. It also is a Toll-like receptor- 3 (TLR3) agonist.Mixtures that included the TLR7/8 agonists R848 or CL075, combined with the poly (I:C) can produce 3-d mature dendritic cells .

Key Properties

• CAS Number: 24939-03-5
• MW: 671.41
• Purity: 90%
• Formula: C₁₉H₂₇N₇O₁₆P₂
• SMILES: O[C@@H]([C@H](N1C(NC=NC2=O)=C2N=C1)O[C@@H]3COP(O)(O)=O)[C@@H]3O.O[C@H]([C@@H](COP(O)(O)=O)O[C@H]4N5C=CC(N)=NC5=O)[C@@H]4O
• Target: TLR
• Solubility: H2O:21.5mg/mL;DMSO:<1 mg/mL

Bioactivity

• In Vivo:
  Animal model: lung tumor-bearing mice. Dosage: 10 μg/mouse. Administration: Intraperitoneal injection (i.p.). Result: Induced a significant decrease in the growth of pulmonary metastases in tumor-bearing mice. Reduced the amount of lung foci to ≈ 40%. Significantly increased BAL fluid cell numbers. Increased the level of INF-γ and IL-17A, decreased the levels of IL-13.Increased TLR3 expression. Animal model: Middle cerebral artery occlusion (MCAO) model mice. Dosage: 1.25 mg/kg. Administration: Intraperitoneal injection (i.p.). Result: Reduced focal cerebral I/R injury. Increased the expression of Bcl2, Hsp27, and Hsp70, decreased Bax expression, and reduced cellular degeneration and apoptosis. Protected against cerebral ischemia and conferred protection against cerebral I/R injury through the downregulation of TLR4 signaling via TLR3.
• In Vitro:
  Cell Cytotoxicity Assay Cell line: 16HBE14o- cells. Concentration: 5 μg/mL. Incubation time: 24 h. Result: Did not lead to significant accumulation of LDH in cell-culture medium

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

TLR