PolQi1

SKU: orb2664202

Description

PolQi1 is a potent and selective DNA polymerase theta (Polϴ) inhibitor with a 2 nM IC50. It is a valuable research tool for studying Polϴ's role in DNA repair, particularly in cancer contexts, and has shown utility in both in vitro and in vivo studies exploring targeted cancer therapies.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 2607139-80-8
• MW: 448.77
• Formula: C₁₈H₁₄ClF₅N₄O₂
• SMILES: C(N(C)C1=C(F)C(Cl)=C(F)C=C1)(=O)[C@H]2N(C(=O)NC2)C3=CC(C(F)(F)F)=CC(C)=N3
• Target: DNA/RNA Synthesis
• Solubility: DMSO:3 mg/mL (6.68 mM)

Bioactivity

• In Vitro:
  Methods: SpCas9-inducible HEK293T cells and hiPSCs were treated with 0.3–10 μM PolQi1 in combination with AZD7648 (1 μM) for 3 hours to evaluate HDR knock-in efficiency and InDel levels. Results: In HEK293T cells, 3 μM PolQi1 increased HDR efficiency by 3.9-fold and reduced InDels by 17.4-fold, with no additional benefit observed at 10 μM. In hiPSCs, HDR efficiency was enhanced by 6.6-fold and InDels were reduced by 2.3-fold.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Compound Identifiers

PubChem CID

156068991