POLQ Antibody

• Catalog Number: orb1249588
• Category: Antibodies
• Description: POLQ Antibody
• Target: POLQ
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Predicted Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: DTHQQLTCSNDES
• UniProt ID: O75417
• Tested applications: ELISA
• Application notes: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments in Human Brain (Cerebellum lysates) and in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: POLQ , polymerase (DNA directed), theta , DKFZp781
• Note: For research use only
• NCBI: NP_955452.3