POLK Antibody

• Catalog Number: orb1257192
• Category: Antibodies
• Description: POLK Antibody
• Target: POLK
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLK (NP_057302.1).
• UniProt ID: Q9UBT6
• MW: Observed: 80kDa/110kDa
• Tested applications: IF, WB
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: POLK, DINB1, DINP, POLQ
• Note: For research use only

Western blot analysis of extracts of various cell lines, using POLK antibody (orb1257192) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using POLK antibody (orb1257192). Green：GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Immunofluorescence analysis of U2OS cells using POLK antibody (orb1257192). Blue: DAPI for nuclear staining.