POLK Rabbit Polyclonal Antibody

SKU: orb1816938

Description

POLK Rabbit Polyclonal Antibody

Research Area

DNA Replication, DNA and RNA

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Equine, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human POLK (1-100/870aa)
• Target: POLK
• Molecular Weight: 99 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

DINP; POLQ; DINB1; DNA polymerase kappa; DINB protein; POLK_HUMAN.

Measured by its binding ability in a indirect ELISA. Immobilized Human POLK protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human POLK Antibody, the EC50 is 199.8 ng/ml.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Prostate tissue lysates, Lane 3: Mouse Cerebrum tissue lysates, Lane 4: Rat Testis tissue lysates, Lane 5: Rat Cerebrum tissue lysates, Lane 6: Human HeLa cell lysates, Lane 7: Human Jurkat cell lysates, Lane 8: Human MOLT4 cell lysates, Primary: Anti-POLK (orb1816938) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 99 kDa, Observed band size: 100 kDa.

Quick Database Links

Gene Symbol

POLK